HA-tagged mICAD-L (12) was amplified by PCR using primers (GGGCCCGGAGCTGGTGCAGGCGCTGGCCGCATCTTTTAC and GGTACCCTACGAGGAGTCTCG), cloned in to the ApaI and KpnI sites from the pNHK12 plasmid (alcohol dehydrogenase We (ADH) promoter: AID-HA-mICAD-I), linearized by MfeI, and built-into Trp1 locus then

HA-tagged mICAD-L (12) was amplified by PCR using primers (GGGCCCGGAGCTGGTGCAGGCGCTGGCCGCATCTTTTAC and GGTACCCTACGAGGAGTCTCG), cloned in to the ApaI and KpnI sites from the pNHK12…

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