Suspensions of bacterias from 4 to 5-time old civilizations were standardized in buffer to get cell concentrations which range from 109 to 101 cfu/ml by checking optical thickness using a spectrophotometer (Thermo Scientific? NanoDrop? Onec, Wilmington, DE) at OD 600 nm
Suspensions of bacterias from 4 to 5-time old civilizations were standardized in buffer to get cell concentrations which range from 109 to 101…