At E16.5, this organisation is already observed in the basal and mid-basal turns of the cochlea, whereas mid-apical and apical (except the apical tip) hair cells become organised by E18.5, because these are the last to mature, albeit the first to exit the cell cycle (Chen and Segil, 1999; Matei et al., 2005). deletions are is considered the top candidate gene because it is consistently haploinsufficient (as part of a larger deletion) in all known cases of WHS (Bergemann et al., 2005; Van Buggenhout et al., 2004). WHSC1 functions as a histone methyltransferase (HMT) to regulate gene expression in both embryonic and adult tissue (Brito et al., 2009; Martinez-Garcia et al., 2011). However, although the activity of WHSC1 is controversial, the consensus is that it catalyses methylation of lysine 36 residues on histone 3 (H3K36me), when presented with nucleosomes, the main components of chromatin (Li et al., 2009; Marango et al., 2008; Wagner and Carpenter, 2012). Like all other H3K36-specific HMTs identified thus far, WHSC1 contains the catalytic SET domain (Wagner and Carpenter, 2012). It also contains the chromatin-binding domain, proline-tryptophan-tryptophan-proline (PWWP), which interacts with H3K36me, a plant homeodomain (PHD) and a high-mobility group (HMG) DNA-binding domain (Wagner and Carpenter, 2012). The HMG domain of WHSC1 can interact with the DNA-binding domain of the androgen receptor (AR) and, in the presence of the ligand, enhances AR-mediated transcriptional activation, thereby implicating WHSC1 in the promotion of prostate carcinogenesis (Kang et al., 2009). WHS is a contiguous gene syndrome in which the deletion size varies among affected individuals, with larger deletions resulting in more severe phenotypes. Prognosis thus depends on the diagnosis: most severe cases are stillborn; 35% die within 2 years, and those who survive into adulthood only make slow but steady progress in growth (Shannon et al., 2001; Zollino et al., 2003). The Kcnj12 major features of the syndrome include a distinctive craniofacial appearance (broad, flat nasal bridge, prominent glabella, short philtrum, micrognathia and ocular hypertelorism), short stature due to growth retardation and global developmental delay, intellectual disability, and seizures. Speech problems, genitourinary abnormalities and other craniofacial manifestations such as proptosis, cleft palate, cleft lip and defective dentition are also common (Battaglia et al., 1999, 2001, 2008; Bergemann et al., 2005; Maas et al., 2008; Shannon et al., 2001; Tachdjian et al., 1992; Van Borsel et al., 2004; Verbrugge et al., Verubecestat (MK-8931) 2009; Zollino et al., 2008). The deletion of is associated with many characteristic WHS features, including Verubecestat (MK-8931) the distinctive facial appearance (Bergemann et al., 2005; Van Buggenhout et al., 2004). mouse mutant phenotypes resemble some WHS phenotypic features in human, including developmental delay, growth retardation, and heart, midline Verubecestat (MK-8931) and craniofacial defects (Nimura et al., 2009). Whereas heterozygous mice are viable and show varying degrees of the WHS phenotype, homozygous mice show more severe phenotype and die shortly after birth (Nimura et al., 2009). Mouse knockout studies associate deletion with seizures and abnormal neuronal activity (Zollino et al., 2003, 2008), whereas dental and cleft abnormalities might be due to loss of function (Nieminen et al., 2003). TRANSLATIONAL IMPACT Clinical issue Wolf-Hirschhorn syndrome (WHS) is a rare genetic disorder in humans that causes severe growth retardation, seizures and characteristic craniofacial defects. Affected individuals can also present with heart defects, cleft lip and/or palate, hearing impairment and eye anomalies. WHS is caused by the partial deletion of the short arm of chromosome 4, which harbours two overlapping critical regions (WHSCR-1 and WHSCR-2) consisting of multiple genes. Phenotypic variability and severity of the syndrome is determined by the Verubecestat (MK-8931) extent of the deletion in these regions. The only gene common to both critical regions is mutant mouse is amongst the few animal models developed for WHS. However, the contribution of individual genes within the WHS critical regions to different phenotypes often cannot be established firmly and, in particular, the causes of sensorineural deafness in WHS have so far not been established. Results In this study, the authors used in auditory hair cell development, particularly during cellular organisation and stereocilia morphogenesis, and in hair cell innervation. These alterations might be responsible for sensorineural hearing loss in human WHS. Furthermore, the results provide new insights into the epigenetic legislation of locks cell polarity and claim that this activity is essential for the agreement of cochlear locks cells and their stereocilia. Because epigenetic adjustments by WHSC1 are reversible, they are great targets for medication therapy in WHS. The symptoms can be characterised by otological manifestations such as for example poorly produced ears (microtia), nystagmus, preauricular cysts or fistula (pits), epicanthal folds, low-set ears, otitis mass media, and hearing reduction (Battaglia et al., 1999, 2001, 2008; Bergemann et al., 2005; Chen et al., 2011; Maas et al., 2008; Shannon et al., 2001; Tachdjian et al., 1992; Truck Borsel et al., 2004; Verbrugge et al., 2009; Zollino et al., 2008). However, the causative gene (or genes) for hearing reduction in WHS is not discovered. Deletion of might donate to hearing reduction: mice are deaf due to disorganisation of cochlear helping cells.