After 1?h incubation, the wells were washed as well as the formazan residue dissolved in DMSO (0
After 1?h incubation, the wells were washed as well as the formazan residue dissolved in DMSO (0.1?ml per good). HMBC). HR-MS: DFS Thermo Scientific spectrometer in a complete scan setting (15C500? 45.5 (HCl3, 0.8)), 99, propane-1,3-diamine (Aldrich 98%), hexane-1,6-diamine (Aldrich 98%), heptane-1,7-diamine (Acros 98%), octane-1,8-diamine (Acros 98%), dodecane-1,12-diamine (ABCR 98%), 4,4-methylenedianiline (ABCR 97%) 4,4-oxydianiline (ABCR 97%). The enumeration of atoms in substances is given to be able to assign the indicators within the NMR spectra and will not coincide using the enumeration of atoms within the IUPAC name (discover Supplementary). The precise rotation is provided in (deg ml)?(g?dm)?1; 9-Methoxycamptothecin concentrations of solutions, in (g) (100?ml)?1. 4.2. General man made process of diimin 2aCe (technique a) To a remedy of (10.68 s (C9H3, C9H3), 0.86 s (C8H3, C8H3), 0.90 s (C10H3, C10H3), 1.12 ddd (H4endo, H4endo, 2 12.3, 13.0, 13.0, 16.9?Hz), 1.73C1.83?m (H4exo, H4exo, 2H12), 1.86 dd (H3, H3 16.9, 12.2, 12.2, 181.43 s (C1, C1), 35.18 t (C2, C2), 43.71 d (C3, C3), 27.34 t (C4, C4), 32.06 t (C5, C5), 53.27 s (C6, C6), 46.69 s (C7, C7), 18.81 q (C8, C8), 19.40 q (C9, C9), 11.28 q (C10, C10), 49.98 t (C11, C11), 31.31 t (C12). ?31 (HCl3, 0.70 s (C9H3, C9H3), 0.87 s (C8H3, C8H3), 0.91 s (C10H3, C10H3), 1.14 ddd (H4endo, H4endo, 2 12.2, 13.0, 16.8?Hz), 1.79 ddddd (H4exo, H4exo 2 12.2, 16.8, 12.0, 12.0, 180.99 s (C1, C1), 35.21 t (C2, C2), 43.71 d (C3, C3), 27.37 t (C4, C4), 32.09 t (C5, C5), 53.23 s (C6, C6), 46.66 s (C7, C7), 18.83 q (C8, C8), 19.40 q (C9, C9), 11.31 q (C10, C10), 52.19 t (C11, C11), 30.44 t (C12, C12), 27.31 t (C13, C13). 18 (HCl3, 0.70 s (C9H3, C9H3), 0.87 s (C8H3, C8H3), 0.91 s (C10H3, C10H3), 1.14 ddd (H4endo, H4endo, 2 12.2, 12.8, 12.8, 16.8?Hz), 1.79 ddddd (H4exo, H4exo, 2 12.2, 16.8, 12.1, 12.1, 180.97 s (C1, C1), 35.21 t (C2, C2), 43.73 d (C3, C3), 27.38 t (C4, C4), 32.10 t (C5, C5), 53.23 s (C6, C6), 46.67 s (C7, C7), 18.84 q (C8, C8), 19.40 q (C9, C9), 11.32 q (C10, C10), 52.26 t (C11, C11), 30.39 t (C12, 9-Methoxycamptothecin C12), 27.40 t (C13, C13), 29.28 t (C14). 20.7 (HCl3, 0.69 s (C9H3, C9H3), 0.86 s (C8H3, C8H3), 0.91 s (C10H3, C10H3), 1.13 ddd (H4endo, H4endo, 2 12.3, 13.0, 13.0, 16.7?Hz), 1.74-1.82?m (H4exo, H4exo), 1.86 dd (H3, H3, 16.7, 12.1, 12.1, 181.00 s (C1, C1), 35.19 t (C2, C2), 43.71 d (C3, C3), 27.35 t (C4, C4), 32.07 t (C5, C5), 53.23 s (C6, C6), 46.66 s (C7, C7), 19.38 q (C8, C8), 18.81 q (C9, C9), 11.29 q (C10, C10), 52.22 t (C11, C11), 30.37 t (C12, C12), 27.33 t (C13, C13), LAMA3 29.32 t (C14, C14). ?32.0 (HCl3, 0.71 s (C9H3, C9H3), 0.88 s (C8H3, C8H3), 0.92 s (C10H3, C10H3), 1.15 9-Methoxycamptothecin ddd (H4endo, H4endo, 2 12.2, 12.8, 12.8, 16.8?Hz), 1.76-1.84?m (H4exo, H4exo), 1.88 dd (H3, H3, 16.8, 12.1, 12.1, 180.98 s (C1, C1), 35.23 t (C2, C2), 43.75 d (C3, C3), 27.40 t (C4, C4), 32.11 t (C5, C5), 53.25 s (C6, C6), 46.68 s (C7, C7), 18.85 q (C8, C8), 19.41 q (C9, C9), 11.33 q (C10, C10), 52.28 t (C11, C11), 30.42 t (C12, C12), 27.41 t (C13, C13), 29.38 t, 29.46 t, 29.49 t (C14, C14; C15, C15; C16, C16). ?32.7 (HCl3, 0.84 s (C9H3, C9H3), 0.95 s (C8H3, C8H3), 1.06 s (C10H3, C10H3), 1.18C1.26?m (H4endo, H4endo), 1.49 ddd (H5endo, H5endo, 2 13.0, 17.7?Hz), 1.74 ddd (H5exo, H5exo, 2 13.0, 17.7, 184.43 s (C1, C1), 36.14 t (C2, C2), 43.71 d (C3, C3), 27.34 t (C4, C4), 31.97 t (C5, C5), 53.81 s (C6, C6), 46.99 s (C7, C7), 18.93 q (C8, C8), 19.45 q (C9, C9), 11.12 q (C10, C10), 150.01 s (C11, C11), 119.37 d (C12, C12), 129.24 d (C13, C13),.
