13C-NMR (100 MHz, CDCl3) 169

13C-NMR (100 MHz, CDCl3) 169.65, 169.34, 166.39, 164.64, 152.09, 150.24, 149.81, 135.18, 133.43, 123.21, 122.91, 121.42, 115.72, 115.50, 114.39, 109.72, 64.43, 60.43, 28.89, 26.18, 25.35, 21.05, 17.91, 14.19. which is far better than XL184. In addition, compound 11i (IC50 = 1.1 M) and 13b (IC50 = 1.2 M) had better results against MCF-7 than XL184 (IC50 = 2.6 M). 2.2.3. Structure Activity Relationship Analysis Preliminary structureCactivity SAR study JW-642 appeared that different head groups showed great differences in cell and enzymatic activity. Among them, quinazolines (compound 9a, 11aCc, 13aCc), and tetrahydro-pyridothienopyrimidines (compound 9c, 11gCi, 13gCi) head groups provided much better activity than pyridines (compound 9b, 11dCf, 13dCf). For the side chain, values were in Hz. Chemical shifts were expressed in ppm downfield from internal standard TMS. HRMS data were obtained using Bruker micro TOF-Q instrument (Bruker) or TOF-MS instrument (Bruker). 3.1.1. General RLC Procedure for the Preparation of 4-Chloro-6,7-dimethoxyquinazoline (Compound 5) 2-amino-dimethyl aminobenzoic acid 2.02 g (10 mmol) and acetic acid formamidine 2.10 g (20 mmol) were added in 2-methoxy ethanol. The mixture was reflux in 125 C. After evaporation of solvent, the residue was added in the 10% ammonia solution, stirring, then after filtering, the solid was washed by water to get the brown solid powder (yield 89%). 1.82 g (8.8 mmol) product was dissolved in 25 mL SOCl2, and 30 drops of DMF was added. After heating 7 h to reflux, the solvent was evaporated, then after filtering, the solid was washed by water to get the brown solid powder 4-chloro-6,7-dimethoxyquinazoline (yield 93%). 3.1.2. General Procedure for the Preparation of {= 7.3 Hz, 1H), 7.79 (s, 1H), 7.64 (t, = 7.8 Hz, 1H), 7.60 (s, 1H), 7.57 (s, 1H), 7.54 JW-642 (s, 2H), 7.52 (s, 2H), 7.44 (t, = 7.8 Hz, 2H), 7.40 (s, 1H), 7.31 (t, = 7.3 Hz, 1H), 6.40 (s, 1H), 4.01(s, 3H), 3.97(s, 3H), 1.31 (s, JW-642 9H). HRMS (= 7.4 Hz, 2H), 7.60 (t, = 8.2 Hz, 1H), 7.53 (s, 1H), 7.47 (t, 2H), 7.33 (s, 1H), 6.99 (dd, = 8.6, 2.3 Hz, 1H), 6.84 (d, = 8.6 Hz, 1H), 4.07 (d, = 2.8 Hz, 6H), 3.90 (d, = 5.2 Hz, 3H), 3.87 (s, 3H). 13C-NMR (100 MHz, CDCl3) 166.79, 165.05, 154.75, 153.84, 153.57, 149.95, 148.67, 148.21, 138.69, 132.68, 129.19, 126.27, 122.57, 122.29, 118.41, 114.98, 113.43, 111.44, 108.20, 107.06, 56.83.HRMS (= 7.8 Hz, 1H), 7.60 (s, 1H), 7.58 (s, 1H), 7.41 (t, 3H), 4.00 (d, = 5.4 Hz, 6H). 13C-NMR (100 MHz, DMSO) 165.19, 162.11, 155.85, 154.74, 153.57, 147.81, 137.54, 136.45, 130.37, 129.25, 128.67, 127.37, 122.88, 122.46, 121.79, 114.79, 110.88, 107.91, 57.24.HRMS (= 2.3 Hz, 2H), 7.79 (q, 1H), 7.67 (t, = 7.8 Hz, 1H), 7.62C7.56 (m, 2H), 7.42 (s, 1H), 7.20 (t, = 7.2 Hz, 2H), 4.00 (d, = 5.2 Hz, 6H). 13C-NMR (100 MHz, DMSO) 167.33, 166.05, 162.07, 159.55, 154.65, 153.84, 153.12, 148.21, 138.69, 134.01, 129.03, 125.77, 122.62, 122.03, JW-642 115.11, 111.14, 108.30, 55.43.HRMS (= 5.6 Hz, 1H), 8.22C7.89 (m, 2H), 7.67C7.37 (m, 2H), 7.34 (s, 1H), 7.27C7.04 (m, 3H), 6.91 (d, = 15.4 Hz, 1H), 6.48 (s, 1H), 3.83 (s, 3H), 3.76 (s, 3H), 2.85 (s, 3H). 13C-NMR (100 MHz, CDCl3) 168.12, 167.12, 164.25, 157.14, 149.75, 148.47, 147.57, 146.98, 135.32, 132.18, 129.23, 123.62, 118.50, 117.10, 113.43, 113.11, 112.47, 107.06, 56.83, 26.37. HRMS (= 5.6 Hz, 1H), 8.01C7.78 (m, 2H), 7.81C7.62 (m, 2H), 7.65C7.42 (m, 2H), 7.48C7.29 (m, 3H), 7.16 (m, 1H), 6.52 (s, 1H), 2.85 (s, 3H). 13C-NMR (100 MHz, CDCl3) 171.77, 169.22, 165.15, 159.14, 149.17, 147.28, 136.46, 135.21, 131.37, 129.44, 127.45, 124.57, 121.78, 118.60, 114.31, 113.17, 24.77. HRMS (= 5.6 Hz, 1H), 8.02C7.76 (m, 2H), 7.72C7.46 (m, 4H), 7.34 (m, 1H), 7.21C7.01 (m, 3H), 6.50 (s, 1H), 2.85 (s, 3H). 13C-NMR (100 MHz, CDCl3) 169.39, 168.32, 167.05, 163.07, 159.55, 158.34, 148.57, 146.98, 135.40, 134.03, 132.01, 128.43, 123.62, 122.66, 121.58, 118.51, 115.10, 113.90, 112.17, 110.47, 28.37. HRMS (= 8.6 Hz, 1H), 3.99 (d, = 4.3 Hz, 1H), 1.62 (s, 1H). 13C-NMR (100 MHz, CDCl3) 172.19, 171.92, 165.66, 162.17, 159.64, 156.11, 155.44, 153.27, 152.81, 150.33,.